FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10673004
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13400.017.1670911
GTATTAG21500.016.448471
TCTATAC23300.016.2094233
GTCCTAG25350.016.1174261
GTGCTAG27600.014.8035061
GTATAGG17800.014.2737621
GTCCTAA23950.013.9057031
GTTCTAG31950.013.8626291
CTATACT27150.013.6580524
GGTCTAG20000.013.3903311
TATACTG32350.013.2672455
GTACTAA18950.013.226361
TCTGTAC44200.013.205583
GTATATA39300.013.1920151
GTATTAA33450.012.9330551
GTCTTAG24550.012.8665781
GTGTATA29300.012.5384221
GTTCTAA30450.012.4031531
TATTAGG24100.012.3946142
GCTCTAG29100.012.3886231
Produced by FastQC (version 0.11.5)