FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10673004
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG26450.016.889831
GTACTAG14100.016.085421
GTATTAG23700.015.804681
GTATTAA28950.015.43129351
GTGCTAG27850.014.6834921
GCGCTAG4750.014.4691821
GTGCTAA25300.013.5827291
CCTATAC17400.013.421173
TCTAGAC25750.013.3368573
CTATACT25900.013.2578764
GCTCTAG31350.013.0441871
GGTCTAA20650.012.9802231
GTATAAG22000.012.8085141
GTCTTAG24150.012.8065741
GTTCTAA29850.012.77868751
TCTATAC23300.012.6757373
GTACTAT22150.012.4114871
GTCTTAA29700.012.3804061
GTTCTAG30200.012.1754341
GTATAGA25450.012.1524081
Produced by FastQC (version 0.11.5)