FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13185351
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA25350.017.4170131
GTATTAG25550.016.7406541
GTCCTAG31450.016.5614341
GTATTAA42050.015.9139291
GTCTTAG32800.015.6694621
GGTCTAA23950.015.5546381
GTGCTAG32800.015.1436411
GTACTAG14950.014.535871
GTTCTAG37750.014.43716051
TAGTGCG6400.014.26044157
GTATAAG26850.014.1315591
GTATACG5150.014.0654531
GCTCTAG37500.013.4296061
GTCTAGA31100.013.4204371
GTATAGG22150.013.3926451
GTGCTAA32800.013.355851
GTCTTAA37800.013.1405141
GTACAGG54850.013.0806221
CTATACT30450.012.8954314
GTGTTAG33850.012.8396621
Produced by FastQC (version 0.11.5)