FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13002417
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25550.017.1073171
GTCCTAG30950.016.7913421
GTACTAG16500.015.4353641
GTATTAA40400.015.0785991
GTCCTAA28800.014.8182961
GTATAGG21750.014.5578631
GTACTAA24350.014.4168471
GTATATA49600.014.15525151
GTCTTAG29450.013.790051
GTGCTAG30050.013.743771
GTCTAGA28100.013.5952051
GTATAAG26600.013.1973771
CGATCGT4509.276846E-1113.006762538
GTTCTAG36900.012.5914721
GCTCTAG37000.012.4644231
GTCTTAA35400.012.4444521
GTACAGG55350.012.4360221
GTACAGA61050.012.40241051
ATTAGAC26100.012.395333
TTATACT44450.012.3886034
Produced by FastQC (version 0.11.5)