FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13002417
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18050.019.8747651
GTATTAG27800.017.6193311
GTGCTAG34400.015.8432611
GTCCTAG33550.015.5249551
GTATTAA37550.015.1572311
GGTCTAA23000.014.9974721
GTACTAA25700.014.3613921
GTCTTAG29900.014.1899171
GTGCTAA31600.014.0814881
GCTCTAG36300.014.0637461
GTATAAG28700.014.0620911
GTATTAT38050.013.8701991
GTTCTAA38850.013.8509471
GTCCTAA29700.013.5886191
GTTCTAG38500.013.4392941
GTATATA52100.013.241531
TATACTG38750.012.8804485
GTATAAA50400.012.7299971
GTGTTAG34350.012.6528881
GTCCTAC29600.012.5857171
Produced by FastQC (version 0.11.5)