FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13125228
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17300.018.1005081
GTATTAG27200.017.0789171
GTATTAA39300.015.9358151
GTGCTAG30450.014.6910051
GTCCTAG32850.014.4557021
GTACTAA24050.014.1649671
GTATATA50800.014.0217461
GTTCTAA38850.013.906061
GTACTAT25350.013.3028151
GTATACG5700.013.2813831
GTATATG37950.013.2384321
GCTCTAG35250.013.1786241
GTCCTAA27950.013.0502711
GTCTTAG32300.012.9972891
TCTAGAC26950.012.8962373
GTTCTAG38500.012.8705531
GGTCTAG26950.012.6407221
GTACAGG55850.012.6306641
GCTCTAA36850.012.6064191
GTATAAA53300.012.5247761
Produced by FastQC (version 0.11.5)