FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13064307
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA41950.018.0529081
GTATTAG27000.016.9568181
GTCCTAG34450.016.4873641
GTACTAG16850.014.7091991
GTGCTAG33200.014.4122971
GTACTAA24200.014.366881
GTCCTAT26250.014.1628581
GTCCTAA26000.013.7694451
GTTCTAG36350.013.7315661
GTATAGG21850.013.7064281
GTCTTAG28700.013.5535481
GTGTTAG36300.013.3711561
GTATATG39000.013.1515871
GCTCTAG35700.013.1137581
GTATATA49500.013.0044761
GTCTATA24400.012.9793951
GTGTATA37200.012.8625881
ACTAGCG3751.6319973E-812.8515823
TTATACT45500.012.7860114
TCTAGAC29300.012.6886653
Produced by FastQC (version 0.11.5)