FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13064307
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28600.016.7348271
GTACTAA25450.016.6413971
GTCCTAG35100.016.578761
GTACTAG16950.016.4546181
GTATTAA39000.015.9803551
CTATACT31600.015.36012654
GTCCTAA29050.014.8161831
GTCTTAA38650.014.6996471
GTGCTAG33200.014.1050071
GTTCTAG36050.014.0405651
GTCTTAG31350.013.7291921
GGTCTAG27250.013.5204071
GTCCTAT26750.013.5156821
GTTCTAA38050.013.4835441
GGTCTAA23400.013.2433891
GTATATA50550.013.0783391
TATACTG40500.013.0052615
TCTATAC28300.012.7722953
GTGTTAA39950.012.2389471
GTATAAG25250.012.1367141
Produced by FastQC (version 0.11.5)