FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11622908
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22250.018.7018551
GTACTAG13850.017.9212781
GTATTAA32650.016.2104341
GTCCTAG29600.016.0310331
GTCTTAG25600.015.3990391
CGATCGT5350.015.00922238
GTTCTAG32900.014.8668421
GTGCTAG29800.014.6985611
GCGATCG6100.013.76218137
GTATTAT33500.013.4020161
GCTCTAG33650.013.2338011
GTCCTAA24650.013.0309351
GTTCTAA32000.013.0036331
GTATAGG16400.012.909041
GTGTTAG28600.012.7627361
CCCCTAG33000.012.7201941
TCTGTAC48050.012.3064123
GGTCTAA21100.012.282471
TATACTG32100.012.2810125
GCCTTAG26750.012.2808531
Produced by FastQC (version 0.11.5)