FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11622908
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195170.16791839013093796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG29050.018.72521
GTCTTAG24950.014.4860831
GTATTAA30150.014.4094031
GTTCTAG30150.014.1672281
CGACGAA23800.013.80293873
TCTATAC26200.013.2352473
GTTCTAA32150.013.1723541
GGTCTAG22700.012.8662051
CCGACGA31750.012.76101673
GACGAAT23800.012.72937673
GTACTAC17350.012.62519551
GTATTAG19100.012.615281
GTACAGG49000.012.516981
GTCTTAA28400.012.46923351
CCTATAC19050.012.4545523
GTCCTAT23950.012.3471251
CTGACGC185450.012.32121473
TGACGCT153300.012.30989673
CTATACT26400.012.3049784
GTGCTAG29400.012.2934621
Produced by FastQC (version 0.11.5)