FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12091884
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14000.019.5552941
GTACTAA21550.016.2612931
GTATTAG21750.015.440441
GTATAGG18100.015.1256431
GTCTTAG26450.014.9049061
GTGCTAG29000.014.4754141
GTATTAA34150.014.1095891
GTCCTAG28050.014.0547151
GTCCTAA26500.014.0502951
GTCCTAT25700.013.3513711
GTACATA28750.013.331611
GTATATG30700.012.9604261
ACGAGAC33500.012.85657373
GTATAAG21350.012.8231441
TATACTG35300.012.8226075
GTTCTAA34550.012.6783971
GTACAAG38650.012.5612621
GCTCTAG36850.012.4814261
GTATATA41150.012.4190781
GCCCTAG31250.012.3818911
Produced by FastQC (version 0.11.5)