FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11896622
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167850.1410904708916531No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG31300.018.563151
GTATTAG20350.016.7000391
GTACTAG13400.015.5442211
GTCTTAG26500.015.3065041
TACGCGA2402.2163513E-615.2199172
GTATTAA34200.015.1726411
GTGCTAG30850.015.043451
CGACGAA26150.014.37806573
GTACTAA23250.014.1455061
GGTCTAA19000.014.0400341
GTCCTAT24500.014.020411
GCTCTAG31250.013.9154061
CCGACGA34950.013.89118873
GACGAAT23150.013.24534473
GTTCTAG33600.012.9421551
CTAGTAC13600.012.8883153
GTCTTAA31200.012.8835941
GTCCTAA26500.012.82436851
GTTCTAA31350.012.7053861
TGACGCT162350.012.68124173
Produced by FastQC (version 0.11.5)