FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12772225
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25000.015.4067171
GTCCTAG32000.015.1003351
GTATTAA37150.014.9863461
GTTCTAG35150.014.1455711
GTACTAA25750.013.8701261
GTATATA46550.013.7654021
GTCTTAG27750.013.2490211
GTATAGA27950.013.1542151
CGATCGT5350.013.08566838
GTCCTAA25700.012.9433871
GTCTATA24000.012.8389311
GTATAAG26900.012.7564921
GGTCTAG25350.012.7077111
GTCCTAT27600.012.6866911
GTACTAG15300.012.5871881
GTACAGG56250.012.5743721
CGAATCG5104.3655746E-1112.3512179
GGTCTAA23850.012.3324221
GTTCTAA36250.012.1708241
GTGCTAG31900.012.074231
Produced by FastQC (version 0.11.5)