FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12772225
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15400.018.4211081
GTATTAG25650.015.565711
GTACTAA24200.015.3405861
GTCTTAG30600.015.1078941
GTTCTAA37900.014.8777791
GTCCTAG31500.014.5650571
GTGCTAG31950.014.3599171
GTCCTAA28100.013.9592841
GTATATA46650.013.7388661
GTGTTAG35000.013.7089421
GGTCTAA23450.013.4416051
GTTCTAG35000.013.1085511
GCCCTAG34250.012.8843181
TCTGACG104600.012.67953270
GCCTTAG28700.012.44714451
GTACAGG51650.012.408871
CTGACGC93100.012.328773570
GGTCTAG25700.012.1285341
GTATTAA35400.012.0700221
GCTCTAG37800.012.0448951
Produced by FastQC (version 0.11.5)