FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12429820
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24700.016.8679521
GTCTTAG29250.016.6380081
GTACTAG15450.016.3161011
GTCCTAG33700.015.6877051
GTACTAA23450.014.1838171
CGATCGT5500.013.99992138
GTTCTAG34900.013.5431821
GTATTAA35000.013.5044871
GTATATA49100.013.4769841
GCGATCG5550.013.24332837
GTGTTAG33250.013.0569711
GTGCTAG33550.013.0445731
GGTCTAG23950.013.0105871
GTCCTAA28050.012.9811411
ATCTCCG125300.012.79296770
GTTCTAA34000.012.5629971
GGATTAG26850.012.5181261
GTCCTAT26300.012.380541
GCTCTAG35350.012.1822661
GTACATA32450.012.0841381
Produced by FastQC (version 0.11.5)