FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12121516
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24750.017.1187921
GTACTAG13050.016.0991761
GTCCTAG30950.015.4996831
GTCCTAA25700.015.1235171
GTGCTAG31200.014.7021091
GTCTTAA31200.014.253191
GTCTTAG29200.013.7904351
GGTCTAA23400.013.7668611
GTTCTAG34750.013.6032261
GTATATA45850.013.4411461
GGTCTAG22750.013.3906241
GTGTTAG33200.013.2890951
GCTCTAG37050.013.1367991
GTATAGG18900.012.7835141
GTGCTAA29600.012.4211141
ATCTCCG126400.012.23840770
GTCCTAT26050.012.23197651
GTACTAA21600.012.1582341
GTATTAA33650.012.0707951
TCTCCGA107500.011.94833370
Produced by FastQC (version 0.11.5)