FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12121516
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25450.016.7750631
GTCCTAG31250.016.0131991
GGTCTAA22650.015.295341
GTGCTAG29900.014.6295491
GTTCTAG32450.013.4799221
GTACTAA22350.013.4652071
GTCCTAA27050.013.4542081
GTATATA42900.013.3776141
GTGTTAG34350.013.3455551
GTCTTAA33850.013.3359251
GTATTAA34700.013.3117931
GCCCTAG30600.012.9225771
TCTGACG103300.012.77382670
GTCTTAG27900.012.66803551
GTTCTAA33400.012.5726521
GCTCTAG34350.012.4286851
GTACTAG13750.012.2160451
TCTAGAC27500.012.08713
GTACTAT22500.011.9756841
TATACTG33950.011.8546255
Produced by FastQC (version 0.11.5)