FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7619484
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15150.017.5322671
GTCCTAG19250.016.8444611
GTACTAA15250.014.250521
GTCTTAG17550.014.1519211
GTATTAA23600.013.593491
GTATAGG12250.013.5165281
GTATTAT24100.013.0251991
CCCCTAG20500.012.9567741
GGTCTAA13800.012.7482661
TATACTG24150.012.7130575
GTCTATA14200.012.6320831
GTACATA21000.012.4840151
TAGACTG20950.012.3496415
GTATATA27250.012.2790631
GTGCATA16650.012.2235621
GTACAGG31800.012.1492911
GTATAAG15950.012.1112051
GTTCTAG21150.012.0692811
GTTCTAA22900.012.0507611
TATGCGC2953.358991E-511.6936323
Produced by FastQC (version 0.11.5)