FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7533392
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG8800.015.7219041
GTCCTAG18500.015.5179421
GTTCTAG20450.014.5456431
GTACTAA16100.014.3938421
GTATTAG14200.014.3711471
GTGCTAG20250.014.3476921
GCTCTAG20750.014.3353441
GTTCTAA22800.013.9566361
GTCTTAG17100.013.7543661
GTACTAC10400.013.3031481
GCCCTAG18350.013.0059121
GTATAGG13100.012.9375661
GTATAAG14050.012.8013211
GTGCTAA17900.012.7531851
GTCTAAG15450.012.53681851
GGTCTAA13350.012.4362011
GTATTAA24500.012.2823351
GTACAGG32950.012.071751
GTACATA19800.012.0534591
GGTCTAG15500.012.0500771
Produced by FastQC (version 0.11.5)