FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7514718
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9700.016.3670981
GTGCTAG19900.015.6089981
GGTCTAA13450.015.3962361
GTATAGG12950.015.1911521
GTCCTAA17450.015.0315491
GTGCTAA18300.014.1447671
GCGTTAG2202.8045915E-514.1190491
GTTCTAA22800.014.0777651
GTCCTAG18400.013.8803211
GTACTAA14700.013.8522481
GTATATA28050.013.78068351
GTATTAG14850.013.2475031
GGTCTAG15500.013.1372951
GTTCTAG20250.013.1235491
GCCCTAG19200.012.9424611
GTATAAG14450.012.8976761
CCCCTAG20950.012.5203121
GTCTTAG17800.012.4092511
GTCTTAA22250.012.2541361
GTACAGG31750.012.1747451
Produced by FastQC (version 0.11.5)