FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13030849
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG42650.018.6603781
GTCCTAG41200.018.0765671
GTACTAG22100.018.0059991
GCGAGAC52900.016.7003673
GTGCTAG40600.016.2755431
GGTCTAG31350.015.9538681
GTTCTAG51950.015.6009271
GAGCGAG57750.014.6662461
GTCTTAG38300.014.5839661
GTATAGG26950.014.2237311
GTATTAA64650.014.0044521
GTACTAA37050.013.8935711
CGAGACT62400.013.631314
AGCGAGA62600.013.5294442
GTCCTAA37650.013.4782291
GTCCAGG98100.013.434491
GCCCTAG39200.013.3178141
GTGCAGG92200.013.0667021
GTACAAG38600.012.9573511
TCTAGAC40300.012.7726323
Produced by FastQC (version 0.11.5)