FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12356973
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG40100.018.5726181
GTCCTAG39500.016.636531
GGTCTAG27700.016.0793111
GTTCTAG47800.016.0390551
GTACTAG22150.015.9876771
GTCTTAG36150.015.9564461
GCGAGAC48850.015.8402483
GTACTAA35100.014.8736061
GCCCTAG34850.014.666031
GTGCTAG38150.014.6414551
GTGTTAG49000.014.3051521
GTACAGG50600.014.2857171
GTGCAGG84200.014.0481871
CTAGACT38200.013.9501914
GTCCTAA35050.013.8532281
CCTAGAC36500.013.6999313
CTAGACC28800.013.687434
GTCCTAT31550.013.5386031
GTATTAA62450.013.5041381
GAGCGAG55750.013.4244441
Produced by FastQC (version 0.11.5)