FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11282162
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19350.019.6217651
GTATTAG35350.019.3124731
GCGAGAC46900.018.3668383
GTACTAA31900.016.0222341
GAGCGAG53300.015.7538371
GTTCTAG43350.015.6642481
GTCCTAG34700.015.4658421
GTGCTAG36350.015.2659861
GTATTAA56050.015.1763031
GTCTTAG31550.015.1585491
GTGCTAA34300.015.1140181
CGAGACT54350.015.0433224
GTCCTAA29950.014.7493971
AGCGAGA53800.014.5864472
GTGCAGG84300.014.5511531
GGTCTAG25650.014.3753941
GTGTTAG41950.014.2724191
GCCCTAG32150.014.19432451
GTGCAGA80300.013.7756731
CTAGACC26050.013.7313614
Produced by FastQC (version 0.11.5)