FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10306375
Sequences flagged as poor quality0
Sequence length79
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC45400.018.8935533
GTATTAG33400.017.9293021
GTGCTAG34700.016.8366811
GAGCGAG49050.016.5264641
CGGTCGA2251.1400516E-616.22262849
GTTCTAG37700.016.0780261
AGCGAGA51450.015.6786452
GGTCTAG23050.015.3662111
CGAGACT52100.015.3428454
GTCTTAG29850.015.2908521
GTACTAG18700.015.2306691
GTATTAA51400.015.2025581
GTCCTAG29250.014.98032951
GTCCTAT24950.014.4887411
GTGTTAG40150.014.1874721
GCCCTAG27050.014.1738551
CAAGACT80950.014.1132484
GCTAGAC24850.014.1008883
GTGCAGG73350.014.0880961
CCAGGAC78250.014.0871843
Produced by FastQC (version 0.11.5)