FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10306375
Sequences flagged as poor quality0
Sequence length79
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19750.021.6200661
CGAGACT39250.017.2009934
GCGAGAC39700.017.1899493
GTCCTAG29850.017.1167771
GTATTAG35400.016.907481
GTTCTAG40050.016.766931
GTCTTAG29050.016.4574831
GAGCGAG47100.016.1943551
GTGCTAG33900.016.148411
GTACGGA5700.015.3664891
GTCCTAA27100.015.3523141
GGTCTAG26400.015.0681811
GTATAAG28800.014.6995411
GTGCAGA64100.014.6323271
GTACAGG39100.014.5608291
GTACTAC18100.014.5175121
GTGCTAA31450.014.5053311
GTCCTAT26000.014.4577981
GCTAGAC23000.014.4389983
CTAGACC23000.014.4389294
Produced by FastQC (version 0.11.5)