FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11116933
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA1503.575196E-416.072272
GTTCTAG31800.015.9206431
GTCTTAG23050.015.3899331
GTCCTAG28650.015.266851
GTACTAG13250.015.0758741
GTATTAG22500.014.6946451
GTCCTAA24050.014.0339971
GTACATA27550.014.0012491
GTACTAA19800.013.9153831
GTATATA37000.013.8693131
TATACTG30000.013.66142755
GTTCTAA30150.013.3649991
GTATTAA32450.013.160651
GTCTTAA29350.013.14257051
CTATACT25800.012.9485884
CTGTACT53550.012.6700144
TCTATAC22050.012.651643
GTCTATA19150.012.4093971
GTGCTAG26100.012.4038861
GTACTAT21550.012.1460961
Produced by FastQC (version 0.11.5)