FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11348776
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG28950.015.4663861
GTACTAG12450.014.6622481
GTATAGG19300.014.4551211
GTCTTAG25950.014.0689781
GTATTAA34100.013.8375841
GTTCTAA32150.013.7127021
GTATTAG21400.013.6804121
GTATAAG21850.013.5562961
GTACTAT21150.013.5164231
ATACGAC4651.0913936E-1113.3312113
CGATCGT3701.3420504E-813.03250938
GTATACG4509.094947E-1113.0115921
GTGCTAG28100.012.9925261
GTCTTAA31300.012.98469451
CTATACT27450.012.7969914
GTACATG42450.012.6572971
GTACTAA21400.012.553791
TCTGTAC48350.012.5362093
TCTATAC25200.012.2996293
GTGTTAG27750.012.2875791
Produced by FastQC (version 0.11.5)