FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11348776
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14350.018.4995121
GTCCTAG29150.017.1494791
GTATTAG22200.017.0828821
GTGCTAG26450.016.2931771
GTATATG33800.014.7901551
GGTCTAG22300.014.5326381
GTTCTAG33900.014.4414261
CTAGACC23050.013.7448064
GTGCTAA24850.013.735051
TCTATAC22200.013.6521543
CTATACT27050.013.4946084
GTCTTAG25500.013.3849411
GTATATA36500.013.3182661
GTATTGG26900.013.2009861
GTCCTAT24600.013.0337481
GTATTAA37850.013.0254151
GTTCTAA32300.013.0220311
GTACTAA20050.012.8963951
GCTCTAG34500.012.791231
CTGTACT53850.012.78986554
Produced by FastQC (version 0.11.5)