FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11115312
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13500.018.370711
GTTCTAG30500.015.4720511
GTCCTAG26350.015.1636781
GCCCTAG24100.015.0071931
GTATAGG18150.014.8028441
GTATTAG23000.014.3770771
GTCTATA21200.014.2979581
GTATTAA34100.013.8386391
GCGATCG3751.0950316E-913.778937
GCGCTAG3901.9717845E-913.2481071
GTATATA41500.013.114031
TACGTCG5003.6379788E-1213.08777528
CTATACT24550.013.0466224
TACGCGA2406.481065E-512.9151042
CTGTACT56900.012.89240554
GTACAGG49450.012.7471171
GTACAAG37100.012.7196111
GTCCTAA23100.012.6745971
GTATTAT31900.012.4175051
TCTAGAC23300.012.4162373
Produced by FastQC (version 0.11.5)