FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11115312
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13150.022.281991
GTCCTAG27850.016.8335041
GTACTAA21800.015.9707641
GGTCTAA21100.015.1936231
GTATTAG21200.014.7967511
GTTCTAG31500.014.6640881
TACGCGA1709.212438E-414.1878442
CTATACT25150.014.1063964
GTCTTAG25650.013.97676851
GTGCTAA25400.013.7071911
GTATTAA32950.013.7049271
GTATATG31950.013.7023061
CTAGACC22650.013.686464
GTCTAGG26800.013.5056451
GTCTTAA30500.013.4495561
TCTAGAC22300.013.4393463
GTATAGA24950.013.2636031
CCTAGAC24950.013.2545333
CGATCGT4701.2732926E-1113.194386538
GTATAGG17100.013.1032211
Produced by FastQC (version 0.11.5)