FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10176865
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21900.017.6671241
ATACGCG1451.7715251E-417.6211453
GTCCTAG25000.014.0163641
TCCGAGC128100.013.96163173
GTACTAG13750.013.5385331
CCGAGCC112450.013.37294273
GTCTTAG22750.012.995941
CGAGCCC85000.012.45283673
GCTCTAG29900.012.4518281
ACGAGAC28200.012.42542673
GGTCTAA21200.011.707851
CTAGACT21900.011.5004114
GTACGAC7350.011.4220653
CTCCGAG161250.011.22719272
GTATTAA32050.011.1609751
GTTCTAG28850.011.1337381
GTCTAGA23400.011.0750731
GTATAGG17900.011.0114581
GGTCTAG19700.010.9317551
GTCCTAT21850.010.8584041
Produced by FastQC (version 0.11.5)