FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10551777
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13000.014.320281
TCCGAGC135250.014.2220173
GTTCTAG31550.013.8837441
GCGCTAG3252.5468034E-713.4779111
GTATTAG22650.013.3762561
CCGAGCC118950.013.19446273
CGAGCCC88600.012.9355173
ACGAGAC29100.012.66823373
TAGTGCG5557.2759576E-1212.49534557
GTATAGG16850.012.13145351
TCTATAC21900.012.0008793
GTGCTAG25600.011.8348511
GTCCTAG24700.011.8227291
GCCCTAG24150.011.6385351
CGATCGT4103.362129E-711.57297738
GCTCTAG31750.011.4969061
GTCTTAG23600.011.4457531
CTCCGAG169850.011.36783572
GTACAGA49400.011.3054851
CTAGACT23250.011.1471064
Produced by FastQC (version 0.11.5)