FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10551777
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA22550.016.18869273
GACGAAT22350.014.86353973
GTACTAG12400.014.7379911
TGACGCT130400.014.613399573
ACGAATA17450.013.38883873
GACGCTG117600.013.37914373
CCGACGA28400.013.36821272
ACGCTGC89400.013.18934873
GTCCTAG26700.012.8678671
GTCTTAG23500.012.598161
CTGACGC156350.012.18795972
GCGCTAA3901.724984E-712.1834051
GGTCTAA22350.011.7745671
CGAATCG4355.70908E-811.7471159
CTAAGCG7200.011.6636924
GTATTAG20350.011.4949071
GTTCTAG29900.011.4907031
GTACTAA21650.011.4799761
GCCTTAG23900.011.4697321
GTCTAGA23700.011.4123031
Produced by FastQC (version 0.11.5)