FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10388197
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCC85700.015.03408973
TCCGAGC130150.013.7415373
GTATTAG23400.013.7293631
CCGAGCC113400.013.38947373
GTCCTAG24700.013.3023731
GTACTAG12300.012.4660181
GTTCTAG30100.012.3713531
GTCCTAA21900.012.0025061
GTCTTAG23500.011.9620711
GTACTAA21400.011.60055351
CTCCGAG165200.011.40047373
GTGCTAG25150.011.3224441
GTCTAGA24550.011.0043341
TCTATAC21600.010.9854033
GGTCTAG20700.010.934651
GTACAGG41200.010.810511
ACGAGAC25800.010.75169973
TCTCCGA175650.010.72222172
GTCCTAT22500.010.7089021
GTGTAGG34500.010.581921
Produced by FastQC (version 0.11.5)