FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10388197
Sequences flagged as poor quality0
Sequence length79
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG124460.1198090486732202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG23300.015.8413511
ACGCTGC87450.014.98494273
GTACTAG10650.014.4120731
TGACGCT124850.014.296864573
CGATCGT4503.45608E-1113.78764238
GTTCTAG32800.013.7043371
CGACGAA20550.013.32201573
GACGCTG115350.013.03767473
CCGACGA25550.012.28649573
CTGACGC151650.012.27575673
TCTGTAC45200.012.1182113
GCGATCG4851.6534614E-912.04014837
CTAGCGC3355.222284E-611.99039653
GTACTGG41600.011.5959761
GACGAAT20950.011.49953773
GGTCTAA18750.011.3045551
GTCTTAG23650.011.2802441
GTTCTAA33000.011.1849541
GTCCTAA24200.011.1748871
GTCTAGA24250.011.1518451
Produced by FastQC (version 0.11.5)