FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11082452
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG23900.015.5847111
CGACGAA27500.015.1313172
CGAATAG15450.015.12014373
GACGAAT26950.015.03379373
GTTCTAG32750.014.38384151
CCGACGA39600.014.28699573
GTATTAG19300.014.1905891
TGACGCT185550.014.04564473
GTCCTAG28200.013.8557841
GACGCTG165900.013.75111973
GTTCTAA32050.013.5586171
ACGAATA20100.013.43816773
GTACTAG13150.012.7740471
CTGACGC226450.012.63711973
ACGCTGC127550.012.53424173
GCTCTAG32900.012.4313581
GTATACG4152.946581E-812.3190261
CGCTATA3608.8047454E-712.1681422
GGTCTAA21700.012.1162781
GTATTAC19750.012.01829051
Produced by FastQC (version 0.11.5)