FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10803343
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17250.017.139851
GTACTAG11250.016.2229041
TCCGAGC187350.014.78689873
ACGAGAC36850.014.06500473
CGAGCCC120800.013.89888473
GGTCTAA20250.013.6993421
CGAGACA32100.013.64473973
CCGAGCC158300.013.32710873
GTCCTAG26900.013.2979571
GTATATA36400.013.136541
GGTCTAG20400.012.5250361
CTATACT25200.012.4569874
GTCTTAG22050.012.4154881
CGATCGT3851.484841E-712.32456638
GTCCTAT24000.012.3192681
GTATAGG15550.012.20631
GTTCTAA30450.012.1072411
GTGCTAG24200.011.915791
CACGAGA47250.011.81894373
CTCCGAG235900.011.7900672
Produced by FastQC (version 0.11.5)