FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10803343
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143760.13306992104203302No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA28350.017.76766473
GACGAAT26150.016.05204873
CCGACGA36750.015.19631973
CGAATCG3854.1836756E-1115.1683889
TGACGCT181850.015.11422973
GTACTAG13350.014.7673041
GTATTAG20250.014.4229371
GTACTAA19050.013.7983211
GTCCTAG23600.013.6131751
ACGCTGC124350.013.38515373
CTAGACT22000.013.272774
GTTCTAG30700.013.1999821
GACGCTG162450.012.96463973
GTGCTAG26250.012.9342841
CTGACGC216800.012.660851572
GTCTTAG25150.012.629031
GTATTAA30300.012.2898431
GCCGACG45350.012.07308672
GTATAGG15650.011.8971961
GCCCTAG25800.011.8863451
Produced by FastQC (version 0.11.5)