FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11271869
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG24300.014.5711521
GTTCTAG30700.014.5060731
TCCGAGC183500.014.38103773
ACGAGAC35800.014.27358173
CCGAGCC157950.014.07296573
GTACTAG13000.013.7588181
GTATTAG19600.013.409261
CGAGCCC116450.013.19564373
GTATTAA31700.013.1272561
GTCCTAG29100.013.0457411
GGTCTAG20050.012.7441851
GTCTTAG23550.012.4001661
CTCCGAG235950.012.18973373
GTCCTAA22300.011.7857181
GCTCTAG32400.011.7170081
GTCTATA21850.011.6943221
CACGAGA43950.011.62671872
GTCTAGG26750.011.326161
GCGCTAG3559.999627E-611.3107851
GGTCTAA21050.011.2717071
Produced by FastQC (version 0.11.5)