FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11051956
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125440.11350027090227287No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAT27800.017.33268773
CGACGAA29500.016.82882173
GTATTAG21100.015.9346181
GTCCTAG26500.015.7215471
TGACGCT183900.014.68881573
GTACTAG13550.014.5643381
GTGCTAG26550.014.1778051
GACGCTG163350.013.9891773
GGTCTAA20800.013.7046371
GTACTAA22850.013.4347431
CCGACGA37000.013.12147872
GCCCTAG24600.013.0732581
GTATTAA30900.012.65498351
GTCTTAG24900.012.6222091
CTGACGC222150.012.55404973
ACGAATA19900.012.29018773
GTTCTAG30950.012.28031
ACGCTGC122450.012.13311873
GTATATA35350.012.0957491
GTCCTAA23300.011.9204861
Produced by FastQC (version 0.11.5)