FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15139281
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36450.015.8950251
GTTCTAG44750.015.6441691
GTATTAG29150.015.26162051
GTCTTAG32600.015.0217411
GTTCTAA45450.014.4926891
GGTCTAG29950.014.3933761
TATACTG43250.014.19335
CTATACT36600.014.1338244
GCTCTAG44950.014.1168441
GTACTAG17950.013.6409131
GTACTAA28450.013.576421
GTACATA41000.013.5422721
CGATCGT5350.013.53855838
TCTATAC32500.013.47627453
GTACAGG70950.013.3668721
GTATTAA43150.013.3470461
GTATATG45500.013.1882551
GTGCTAA37100.013.1067411
GTATAAG30250.012.9965761
GTGCTAG34000.012.9831651
Produced by FastQC (version 0.11.5)