FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15139281
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19650.017.9553991
GTACTAA30250.017.4953841
GTCCTAG36600.017.2952751
GTATTAA43200.017.0550611
GTATTAG29450.015.7389921
GTCTTAG34550.014.6171321
GTTCTAA45650.014.4727131
GCTCTAG42550.014.3890161
GGTCTAA28050.014.058191
GTTCTAG43550.013.9791861
CTATACT36950.013.8240724
GTATATA49550.013.7524471
GTACTAT27050.013.5548911
CTAGACT34700.013.4274334
GTCCTAA32100.013.3620811
GTGCTAG37850.013.3427211
TATACTG45650.013.2988825
GTACAGA70950.013.2609281
CCTAGAC35000.013.123583
GTACATA41750.012.924851
Produced by FastQC (version 0.11.5)