FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15049361
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157430.10460909270499923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27550.018.7130491
GTACTAG19850.017.6051921
GTACTAA30650.015.4657141
GTGCTAG37350.015.3779121
GTCCTAA34850.014.5946751
GTCTTAA41050.014.5818571
GTCCTAG37350.014.1736171
GTCTTAG34800.014.1185131
GTATTAA44150.014.0282081
GTATATA50100.013.9506211
GCTCTAG45050.013.82475951
GTTCTAA42900.013.7917281
GGTCTAG27600.013.7899741
CTATACT34800.013.787884
GTTCTAG42300.013.5783691
GTATAGG23650.013.3134351
TCTATAC30450.013.2752273
TCTGTAC63450.013.17732053
GTATATG43400.013.1544921
ACTATAC26850.013.1249983
Produced by FastQC (version 0.11.5)