FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15302773
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16950.016.6850361
GTCCTAG38300.016.6592711
GTATTAG29750.015.7664881
GTACTAA29050.015.077891
GTCTTAA41900.014.7340451
CTATACT34750.014.19123654
GGTCTAG28500.014.0377061
GTTCTAG43250.013.4767011
GTCTTAG31700.013.3822421
GTATAAG32200.013.2815511
GTATATA49700.013.2543971
GTATTAA42250.013.2242571
GCTCTAG46650.013.0859311
GTCCTAA31700.013.0558451
TATACTG43650.013.035835
CCTATAC24750.012.95826153
GTACAGG68500.012.8893921
TCTGTAC66150.012.563953
GTACCGT9150.012.4374656
GTACTAT29550.012.37175751
Produced by FastQC (version 0.11.5)