FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15302773
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG38100.018.7531471
GTACTAG18800.017.4419291
GTACTAA30000.016.6830521
GTCCTAA34350.015.8766591
GTGCTAG38350.015.6607531
GTTCTAG43650.015.3407381
GTATTAG29000.014.8778711
GTATAGG26650.014.76510051
GTCTTAG34300.014.5915931
GTTCTAA45200.014.1273961
GTCCTAT35000.013.9052831
GTATATA49800.013.7928031
GTATTAT42650.013.4343871
GGTCTAG29850.013.4135111
CTATACT34750.013.3959874
GTATTAA47450.013.3847541
CGAATCG6700.013.3809159
GCGCTAG5750.013.2063741
TCTAGAC34900.013.1417063
TATACTG43600.013.1284815
Produced by FastQC (version 0.11.5)