FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15030422
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA30300.017.4154681
GTATTAG28950.017.2745111
GTCCTAG34800.016.5509741
GTACTAG18800.015.96054651
GTCTTAG33400.015.1794911
GTATTAA42950.014.6951581
GTCCTAA31850.014.077331
GTATACG6400.014.0113421
GTTCTAA41950.013.9766571
GTATAGG24800.013.9070391
GTACTAT26900.013.8470681
GTACATA42400.013.828321
GTTCTAG43150.013.8277541
GTACAGG64900.013.6576131
GTGTACG13650.013.3915111
GTGCTAG34350.013.3539951
GTATATA55100.013.3325841
GGTCTAG28400.013.2371521
TATACTG45700.013.2054525
GTCTTAA40800.013.1871451
Produced by FastQC (version 0.11.5)