FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11092679
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG26250.016.0074941
GTATTAG19900.015.4038671
GTTCTAG31350.014.5020441
GTATTAA30250.014.3462361
TATACTG32050.014.0779075
GCGCTAG5150.014.0444561
GCCCTAG26050.014.0149321
GTACTAG12400.013.8880471
GTGCTAG25850.013.7236451
CTAGACT22350.013.5611954
GTACAGG52150.013.5391811
GTCTAGG25900.013.431191
GTATATA34800.012.9653691
TAGTGCG5750.012.81957157
TCTGTAC43550.012.8119663
CTATACT25700.012.731614
TCTAGAC23550.012.7238133
GTCTTAG23300.012.7126291
GTATAAG20400.012.6626311
GTCTAGA26000.012.5847071
Produced by FastQC (version 0.11.5)