FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10938297
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG20700.017.004641
GTCCTAG27750.016.0422171
GTTCTAG30950.015.8330761
GTACTAA21500.014.9273291
GTCCTAA21750.014.9144141
GTATTAA30850.014.8776431
GTACTAG12550.014.8486741
CTATACT25200.014.7604024
GGTCTAA20650.014.7061921
CGATCGT4303.6379788E-1214.41944638
GTATACG5050.014.35045051
GCGATCG4900.014.05836237
GTCTTAG24050.013.7750691
GCGCTAG4304.1836756E-1113.6432581
GTGCTAG26850.013.4953011
GTGTTAG26550.013.3878341
GTATAAG19400.013.34126951
GTATATG28700.013.2266041
GTTCTAA30900.013.1783531
TCTAGAC24050.013.0359393
Produced by FastQC (version 0.11.5)