FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10783030
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG25500.017.8403341
GTTCTAG28500.015.9624041
GTGCTAG25050.015.6843441
GTACTAA18600.015.0086381
GTATTAG19050.014.8350191
GTACTAG12200.014.40721
GTATAGG16400.013.6596241
GTTCTAA30800.013.4276421
GCTCTAG32550.013.3410121
GTCTTAG23200.013.2212121
GGTCTAA18700.013.0853691
TACGCGT1850.001752855813.0283234
GTCCTAT23550.013.0247191
CTTAACG7150.013.0029553
GTCCTAA23650.012.9696461
TCTAGAC22600.012.95074753
CTATACT23300.012.8565834
GTATTAA29550.012.8293431
TATACTG30400.012.572215
GGTCTAG21200.012.5176871
Produced by FastQC (version 0.11.5)