FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10633963
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG26000.016.4403691
GTGCTAG26600.016.1991271
GTATTAA29300.016.1181871
GTTCTAG29100.015.5182071
GCTCTAG30200.015.2954081
GTATAGG16700.014.6556511
GTACTAG11000.014.4154571
GGTCTAG19550.014.282411
GTTCTAA30000.014.2483211
GTACTAA19950.013.3048821
GTATTAG20850.013.2265671
GTATATA35250.013.2019431
GTACAAG37550.013.0359181
GTCTAGA24650.013.0055661
GTACATA27250.012.9031851
GTACAGG52100.012.9020921
GCTCTAA31400.012.8445651
TCTGTAC43900.012.7909963
GTCCTAC24850.012.7621741
CGAATCG5155.456968E-1212.7070269
Produced by FastQC (version 0.11.5)